Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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Volume 23

April - June 2007 (Part-I)

Number 2


 

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Evaluation of Anti-ds DNA antibodies in Anti-Nuclear
antibody positive Omani patients

Adel Alnaqdy1, Juma Al-Busaidy2, Batool Hassan3

ABSTRACT
Objective: To study the correlation between enzyme linked immunosorbent assay (ELISA) and immunofluoresencent (IF) anti-dsDNA antibody measurement in different diseases.
Patients and Methods: One hundred and forty sera from patients with systemic lupus erythematosus (SLE, n=40), rheumatoid arthritis (RA, n=30), disease control (n=40) and from healthy control subjects (n=30) were included.
Results: Using the ELISA, serum anti-dsDNA was detected in 24/40 (60%) SLE, 5/30 (16.6%) RA, 9/40 (22.5%) disease controls and 1/30 (3.3%) of normal healthy controls. When IF assay was employed, anti-dsDNA antibodies were detected in 16/40 (40%) SLE, 3/30 (10%) RA, 5/40 (12.5%) disease controls but none in the normal subjects.
Conclusion: These results suggest that both ELISA and IF techniques can be used for the measurement of anti-dsDNA antibodies in clinical laboratories and, these antibodies are not unique to SLE.

KEYWORDS: Anti-dsDNA antibodies, ELISA, Crithidia luciliae, Systemic lupus erythematosus, Heumatoid arthritis.

Pak J Med Sci   April 2007   Vol. 23 No. 2   211-215


1. Dr. Adel Alnaqdy
Department of Microbiology & Immunology
2. Dr. Juma Al-Busaidy
Department of Microbiology & Immunology
3. Dr. Batool Hassan
Department of Medicine
1-3: College of Medicine and Health Sciences,
Sultan Qaboos University, Oman.

Correspondence:

Dr. Adel Alnaqdy,
Department of Microbiology & Immunology
College of Medicine and Health Sciences,
Sultan Qaboos University,
P.O. Box: 35, Al-Khod – 123,
Oman.
E-Mail: aalwan@squ.edu.om

* Received for Publication: July 29, 2006
* Accepted: November 15, 2006


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