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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 25 |
April - June 2009 (Part-I) |
Number 2 |
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Myocardial protection by Ethyl-Isopropyl amiloride,
a specific Na+-H+ exchange inhibitor,
following Hemorrhagic Shock
Mona Soliman1, Abdul-Majid Al-Drees2
ABSTRACT
Background: Hemorrhagic shock and resuscitation is well known to result in myocardial dysfunction and injury. Stimulation of the Na+-H+ exchanger plays an important role in the pathway of myocardial injury. The purpose of the present study was to examine the protective effects of blocking the cardiac Na+-H+ exchange, using 100mM ethyl-isopropyl amiloride (EIPA), a specific Na+-H+ exchanger blocker, on myocardial contractile function on ex vivo resuscitation of isolated rat heart following one hour of hemorrhagic shock.
Methodology: Sprague- Dawley rats were assigned to hemorrhage, hemorrhage + EIPA, sham hemorrhage and sham hemorrhage + EIPA groups. Rats were hemorrhaged for one hour. Hearts were harvested and ex vivo treated and resuscitated by perfused in the Langendorff System. Myocardial function was determined.
Results: The results showed that inhibition of the Na+-H+ exchanger using EIPA improved the post-resuscitation myocardial contractile function.
Conclusion: Blocking the Na+-H+ exchanger using 100mM EIPA following 60 minutes of hemorrhagic shock improved myocardial function.
KEY WORDS: Hemorrhage, Rat, Isolated heart, Contractility, Ethyl-Isopropyl Amiloride, Langendorff.
Pak J Med Sci April - June 2009 Vol. 25 No. 2 289-292
How to cite this article:
Soliman M, Al-Drees AM. Myocardial protection by Ethyl-Isopropyl amiloride, a specific Na+-H+ exchange inhibitor, following Hemorrhagic Shock. Pak J Med Sci 2009;25(2):289-292.
1. Dr. Mona Soliman, MBBS, MSc, PhD
Assistant Professor
2. Dr. Abdul-Majid Al-Drees, PhD
Assistant Professor
1-2: Department of Physiology
College of Medicine
King Khalid University Hospital
Riyadh - Saudi Arabia.
Correspondence
Dr. Mona Soliman, MBBS, MSc, PhD
Assistant Professor, Dept. of Physiology,
College of Medicine,
King Khalid University Hospital,
P.O. Box 2925 (29),
Riyadh – 11461,
Saudi Arabia.
E-Mail: monaslmn@yahoo.com
* Received for Publication: June 26, 2008
* Revision Received: January 21, 2009
* Revision Accepted: January 25, 2009
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