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Volume 26 |
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Number 2 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 26 |
April - June 2010 |
Number 2 |
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Antibacterial agents from the seeds of
Peucedanum zenkeri L. (Umbelliferae)James A. MBAH1, Donatien GATSING2, Simon M.N. EFANGE3
Abstract
Objective: In a search for natural substances with potential for the treatment of typhoid fevers and urogenital infections, the methylene chloride extract of the seeds of Peucedanum zenkeri was investigated.
Methodology: The extract was subjected to column chromatography leading to the isolation of seven compounds. Their structures were determined using modern 2D NMR techniques and by comparison with published NMR data. These compounds were tested against Salmonella typhi, Salmonella paratyphi B, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus, using both agar diffusion and broth dilution techniques.
Results: The compounds isolated were umbelliprenin (1), prangenin (2), imperatorin (3), isopimpinellin (4), bergapten (5), cnidilin (6) and stigmasterol (7). Among the above seven compounds, only two (1 and 2) exhibited antibacterial activity. For compound 1, the MIC value was 300 µg/ml against P. aeruginosa, S. aureus, S. typhi and S. paratyphi B. For compound 2, the MIC values varied between 200 and 300 µg/ml against all the bacteria strains tested.
Conclusion: These data suggest that Peucedanum zenkeri seed extract contains antibacterial agents which are active against Salmonella species causing typhoid and paratyphoid fevers, and some bacteria strains causing urogenital infections. The antibacterial activity of compound 2 appears to be due to the epoxide group present in its structure.
KEY WORDS:
Peucedanum zenkeri, seeds, coumarins, antimicrobial activity, typhoid fever, urogenital infections.Pak J Med Sci April - June 2010 Vol. 26 No. 2 314-318
How to cite this article:
MBAH JA, GATSING D, EFANGE SMN. Antibacterial agents from the seeds of Peucedanum zenkeri L. (Umbelliferae). Pak J Med Sci 2010;26(2):314-318.
1. Dr. James A. MBAH,
2. Dr. Donatien GATSING,
Department of Biochemistry, Faculty of Science,
University of Dschang,
P.O. Box 67 Dschang, Cameroon
3. Prof. Simon M.N. EFANGE,
1, 3: Department of Chemistry, University of Buea,
Box 63 Buea, Cameroon
Correspondence:
Dr. Donatien GATSING,
Department of Biochemistry, Faculty of Science,
University of Dschang, P.O. Box 67 Dschang,
CAMEROON
E-mail: gatsingd@yahoo.com
* Received for Publication: August 20, 2009
* Revision Received: January 29, 2010
* Revision Accepted: January 31, 2010
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