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Volume 23 |
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Number 3 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ORIGINAL ARTICLE- |
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Volume 23 |
April - June 2007 (Part-II) |
Number 3 |
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In vitro activities of amphotericin-B in combination
with rifampin against Aspergillus species
Majid Zarrin1, Mohamed Reza Najafi2
ABSTRACT
Objective: The main goal of study was finding the synergism effect of amphotricin B (AMB) and rifampin (RIF) on 3 species of Aspergillus.
Methodology: Activities of amphotericin B in combination with rifampin were tested in buffered yeast-nitrogen base using checkerboard method. Plates were inoculated with 20µl spores suspensions of each organism and incubated at 30°C for 24h. For this method, the MICs were defined as the lowest antimicrobial concentration inhibiting visible fungal growth on the plates. Minimal fungicidal concentration was defined as the first tube showing no growth on the plate.
Results: The MIC of amphotericin B for 100% of isolates of A. fumigatus and A. flavus were inhibited by 4mg/lit amphotericin B. 100% of isolates of A. niger were inhibited by 8mg/lit amphotericin B. When amphotericin B was combined with rifampin, amphotericin B MICs decreased to 2, 1 and 4mg/lit in A. fumigatus, A. flavus rephrase and A. niger respectively.
Conclusion: The results indicate that combination of amphotreicin B and rifampin was synergistic on A. fumigatus, A. flavus and A. niger.
KEY WORDS: Aspergillus, Amphotreicin B, Rifampin, Synergism.
Pak J Med Sci May - June 2007 Vol. 23 No. 3 323-325
1. Dr. Majid Zarrin
2. Dr. Mohamed Reza Nagafi
1-2: Department of Medical Parasitology and Mycology,
Jundi shapour University of Medical Sciences,
Ahwaz – Iran.
Correspondence
Dr. Majid Zarrin,
Email: mjzarrin@yahoo.co.uk
* Received for Publication: September 8, 2007
* Accepted: December 28, 2006
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