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Volume 25 |
April - June 2009 (Part-II) |
Number 3 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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Volume 25 |
April - June 2009 (Part-II) |
Number 3 |
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Antimicrobial activity by Trigona laeviceps
(Stingless Bee) honey from Thailand
Chanpen Chanchao1
ABSTRACT
Objective: To determine the key properties of Trigona laeviceps honey from Thailand, including its antimicrobial activity.
Methodology: Proline content and the percentage of invert sugar were evaluated as described. Major protein bands were resolved and analyzed by SDS PAGE and MALDI TOF analyses. Antimicrobial activity was assayed by agar well diffusion.
Results: The honey was acidic (pH 3.37) but probably undoctored (proline content of 1,723mg/kg) with a normal sugar content (e.g. invert sugar 15.2% (w/w)) but a higher than expected total protein content (0.28g/100g). From some ten distinct protein bands, six major protein bands were revealed of which the 29 kDa band was likely to be pollen allergen Lol p VA precursor Lolium perenne (Perennial ryegrass). Neat honey is the most effective for use as a contact antimicrobial agent, and Staphylococcus aureus was the most susceptible tested pathogen to honey at all dilutions.
Conclusion: The studied honey could perhaps be used as an antimicrobial agent. Since pollen allergen protein was found, it may cause honey intoxication.
KEYWORDS: Trigona laeviceps, Honey, Proline, Invert sugar, Major protein, Inhibition zone.
Pak J Med Sci April - June 2009 (Part-II) Vol. 25 No. 3 364-369
How to cite this article:
Chanchao C. Antimicrobial activity by Trigona laeviceps (stingless bee) honey from Thailand. Pak J Med Sci 2009;25(3): 364-369.
Dr. Chanpen Chanchao
Department of Biology,
Faculty of Science,
Chulalongkorn University,
Bangkok 10330, Thailand.
Correspondence
Chanpen Chanchao,
E-mail: chanpen@sc.chula.ac.th
* Received for Publication: January 16, 2009
* Revision Received: May 22, 2009
* Revision Accepted: May 25, 2009
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