|
|
||||
|
Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
||||
|
ISSN 1681-715X |
||||
|
||||
|
- |
||||
|
ORIGINAL ARTICLE |
||||
|
- |
||||
|
Volume 23 |
July - September 2007 |
Number 4 |
||
|
|
||||
|
||||
|
|
||||
|
Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
||||
|
ISSN 1681-715X |
||||
|
||||
|
- |
||||
|
ORIGINAL ARTICLE |
||||
|
- |
||||
|
Volume 23 |
July - September 2007 |
Number 4 |
||
|
|
||||
|
||||
Bacteriologic findings in patients with ocular infection
and antibiotic susceptibility patterns of isolated pathogensAzar D. Khosravi1, Manigeh Mehdinejad2, Mahvash Heidari3
ABSTRACT
Objective: Isolation of common bacterial pathogens involved in ocular infection and their in vitro susceptibility to commonly used ocular antibiotics and the trends in antibiotic resistance developed by these pathogens.
Patients & Methods: Corneal scrapings were obtained from 318 hospitalized patients through Central laboratory, Imam Khomeini Hospital, Ahwaz, Iran from October 2005 to March 2006.They were inoculated directly onto blood agar and thioglycollate broth and were incubated. The isolates were identified as per standard procedures. Antibiotic susceptibility was determined for all positive cultures and susceptibility of isolated pathogens to commonly used ocular antibiotics was examined.
Results: Of the isolats (n=70) Gram positive cocci accounted for 47 (67.2%) of all bacterial isolates gram negative bacilli for 23 (32.8%). Coagulase negative staphylococci (33%) and Pseudomonas sp. (24%) were most common isolated. In susceptibility testing, Gentamicin had coverage against 35 (74.5%) of 47 gram positive cocci and 19 (82.6%) of 23 gram negative isolates. The coverage of Tetracycline, Cephalotin and Ceftriaxon against the gram positive cocci isolates were 61.7%, 55% and 53% respectively. All the gram positive cocci isolats showed resistance to Cefotaxime and Penicillin. Ceftriaxon and Tobramycin had coverage against 17 (73.9%) and 14 (60.8%) of 23 gram negative isolates respectively. The coverage of Vancomycin against coagulase negative staphylococci was 100%. However all the isolates of S. aureus were resistant to Vancomycin.
Conclusions: Susceptibility analysis revealed that the most effective antibiotics were Gentamicin (77.1% of 54 isolates) and Ceftriaxon (42% of 42 isolates). Both antibiotics had good coverage against gram-positive cocci which constitute the majority (67.1%) of ocular isolates in this study.
KEY WORDS: Corneal pathogens, Antibiotic, Susceptibility test.
Pak J Med Sci July - September 2007 Vol. 23 No. 4 566-569
1. Azar D. Khosravi Ph D,
Dept. of Microbiology, School of Medicine,
2. Manigeh Mehdinejad
Infectious and Tropical Diseases Research Center,
1-2: Ahwaz Jondishapour University of Medical Sciences,
Ahwaz – Iran.
3. Mahvash Heidari
Central Laboratory, Imam Khomeini Hospital,
Ahwaz – Iran.
Correspondence
Dr. Azar D. Khosravi,
E-mail: khosraviaz@yahoo.com
* Received for Publication: December 14, 2006
* Accepted: April 3, 2007
HOME | SEARCH | CURRENT ISSUE | PAST ISSUES
Professional
Medical Publications
Room No. 522, 5th Floor, Panorama Centre
Building No. 2, P.O. Box 8766, Saddar, Karachi - Pakistan.
Phones : 5688791, 5689285 Fax : 5689860
pjms@pjms.com.pk
