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Volume 24 |
July - September 2008 |
Number 4 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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Volume 24 |
July - September 2008 |
Number 4 |
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Nested PCR based diagnosis of Salmonella enterica
serovar Paratyphi A directly from blood samples
Aamir Ali1, Abdul Haque2, Yasra
Sarwar3, Asma Haque4, Mashkoor Mohsin5,
Amna Afzal6, Tayyaba Iftikhar7, Ayesha Tariq8
ABSTRACT
Objective: Development of a rapid, reliable PCR - based method for molecular identification of Salmonella enterica serovar Paratyphi A directly from blood samples.
Methodology: S. Paratyphi A isolates were used for regular PCR targeting specific region of fliC-a gene. New primers were designed and conditions were optimized for a nested PCR that could be directly applicable on blood samples. The procedure was tested on 70 blood samples from suspected cases of typhoidal infection and comparison made with blood culture.
Results: Blood culture was able to diagnose only four patients as infected with S. Paratyphi A. Regular PCR was unable to detect S. Paratyphi A directly from blood where as nested PCR detected S. Paratyphi A in blood of thirteen patients.
Conclusions: S. Paratyphi A, which is emerging as a major pathogen can be detected with better sensitivity by nested PCR as compared with blood culture.
KEY WORDS: Salmonella Paratyphi A, Nested PCR, Paratyphoid Diagnosis.
Pak J Med Sci July - September 2008 Vol. 24 No. 4 545-549
How to cite this article:
Ali A, Haque A, Sarwar Y, Haque A, Mohsin M, Afzal A, et al. Nested PCR based diagnosis of Salmonella enterica serovar Paratyphi A directly from blood samples. Pak J Med Sci 2008;24(4):545-9.
1. Aamir Ali, D.V. M., M. Phil.
(Biotech)
2. Abdul Haque, M. Sc. (Med. Micro), Ph. D. (Med Mol. Biol)
3. Asma Haque, M. Sc. (Chem), M. Phil. (Chem)
4. Yasra Sarwar, M. Phil. (Biotech)
5. Mashkoor Mohsin, M. Phil. (Biotech)
6. Amna Afzal, M. Sc. (Biochem)
7. Tayyaba Iftikhar, M. Sc. (Zoology)
8. Ayesha Tariq, MBBS, M. Phil. (Biotech)
1-8: Health Biotechnology Division,
National Institute for Biotechnology and
Genetic Engineering (NIBGE),
Jhang Road, Faisalabad, Pakistan.
Correspondence
Dr. Abdul Haque
Principal Scientific Officer, Health Biotechnology Division,
National Institute for Biotechnology and
Genetic Engineering (NIBGE), P.O.Box 577, Jhang Road,
Faisalabad, Pakistan.
E-mail: ahaq_nibge@yahoo.com
abdulhaq@nibge.org
* Received for Publication: March 20, 2008
* Revision Received: May 23, 2008
* Revision Accepted: May 24, 2008
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