Pakistan Journal of Medical Sciences

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ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 25

July - September 2009

Number  4


 

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Comparative Study of Aminoglycosides (Gentamicin & Streptomycin)
and Fluoroquinolone (Ofloxacin) Antibiotics on Testis Tissue in Rats:
Light and Transmission Electron Microscopic Study

Arash Khaki1, AmirAfshin Khaki2, Sohrabihaghdost Iraj3, Parviz Bazi4,
Seyed Amir Mahdi Imani5, Homan Kachabi6

ABSTRACT

Objective: To investigate the comparative effects of aminoglycosides and fluoroquinolones on testis structure and serum testosterone hormone level in rats.

Methodology: Forty male Wister rats were randomly divided into control (n=10) and experimental (n=30) groups. The experimental groups were subdivided into three groups of ten. Each received 5 mg/kg (IP) Gentamicin, 40mg/kg (IP) Streptomycin and 72mg/kg (IP) Ofloxacin daily for 14 days, respectively; however, the control group just received vehicle (IP). In the fourteenth day, 5cc blood was collected for testosterone hormone then rats were killed and testis tissues were also prepared for light and electron microscopic study.

Results: Depletion of germ cells, germinal cells necrosis, especially in spermatogonia, and Leydig cells had an abnormal fibroblast-like appearance. Abnormal space between neighbour sertoli cells, mitochondria were lost cristae and vacuolated (none energized), lyzosome seen more in cytoplasm of sertoli cells and Veins congestion were seen in gentamicin & ofloxacin groups. These side effects were seen fewer in Streptomycin group.

Conclusions: Gentamicin, Streptomycin and Ofloxacin have negative effects on testis architecture and germinal cells damages in rats. However, these side effects are seen less in the Streptomycin group. Therefore, it is recommended that usage of this drug have fewer side effects on male fertility.

KEY WORDS: Gentamicin, Streptomycin, Ofloxacin, Testosterone, Testis.

Pak J Med Sci    July - September 2009    Vol. 25 No. 4    624-629

How to cite this article:

Khaki A, Khaki AA, Iraj S, Bazi P, Imani SAM, Kachabi H. Comparative Study of Aminoglycosides (Gentamicin & Streptomycin) and Fluoroquinolone (Ofloxacin) Antibiotics on Testis Tissue in Rats: Light and Transmission Electron Microscopic Study.. Pak J Med Sci 2009;25(4):624-629.


1. Arash Khaki,
Department of Veterinary Pathology (YRC),
2. AmirAfshin Khaki,
Department of Anatomical Science,
Tabriz University of Medicine Science,
National Management Center for Health [NPMC],
Tabriz, Iran.
3. Sohrabihaghdost Iraj,
Department of Veterinary Pathology,
Islamic Azad University Sciences,
Research Branch, Tehran - Iran.
4. Parviz Bazi,
Department of Anatomical Science,
Boushehr University of Medicine Science.
5. Seyed Amir Mahdi Imani,
Department of Veterinary Pathology (YRC),
6. Homan Kachabi.
Department of Veterinary Pathology (YRC),
1,4-6: Islamic Azad University Tabriz Branch,
Iran.

Correspondence

Arash Khaki
Department of Veterinary Pathology (YRC),
Islamic Azad University Tabriz Branch,
Tabriz - Iran.
E-mail: arashkhaki@yahoo.com

* Received: October 7, 2008
* Revision Received: June 11, 2009
* Revision Accepted: July 8, 2009



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