Pakistan Journal of Medical Sciences

Published by : PROFESSIONAL MEDICAL PUBLICATIONS

ISSN 1681-715X

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ORIGINAL ARTICLE

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Volume 22

October - December 2006

Number 4


 

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Evaluation of the antibacterial activity of the seed hull
of Quercus brantii on some gram negative bacteria

Azar D. Khosravi1, Atoosa Behzadi2

ABSTRACT

Objective: Due to rapid increase of antibiotic resistance especially among gram negative bacteria that cause diarrheal diseases, the role of methanol extract of oak seed hull (Quercus brantii) was evaluated on few gram negative entric bacilli and compared with some in-use antibiotics.

Duration and place of study: Nine months from October 2001 to July 2002 at the schools of Medicine & pharmacy, Ahwaz Jondi Shapour University of Medical Sciences, Ahwaz, Iran

Metrials & Methods: Cold maceration with 70% methanol was used for extraction of seed hulls and different concentrations were prepared from the concentrated extract. The antimicrobial activity of the extract was examined using the standard MIC and disc diffution method on E. coli, Salmonella typhimurium, Shigella flexneri and Proteus mirabilis and the activity was compared with those of gentamycin, nalidixic acid and co-trimoxazole in the next step.

Results: The antibacterial effect of the methanol extract on P. mirabilis and E. coli was significant and was directly concentration- related but had no significant effect on S. flexneri. Some concentrations had a similar or even better effect compared with nalidixic acid or co-trimoxazole, while the effect of 80% extract was not significant in general, except for S. typhimurium where its effect was equivalent to that of 25g co-trimoxazole.

Conclusions: Although oak seed hull has some antibacterial activity apparently its anti-diarrheal effect is due to water absorption and protein precipitation in the body.

KEY WORDS: Seed hull, Antibacterial activity, Quercus brantii, Salmonella, Shigella, E. coli.

Pak J Med Sci October - December 2006 Vol. 22 No. 4 429-432


1. Dr. Azar D. Khosravi,
Department of Microbiology,
School of Medicine& Infectious and
Tropical Diseases Research Center.

2. Dr. Atoosa Behzadi,
School of Pharmacy.

1-2: Ahwaz Jondi Shapour University of Medical Sciences,
Ahwaz, IRAN.

Correspondence:
Dr. Azar D. Khosravi
E-Mail: khosraviaz@yahoo.com

* Received for Publication: December 6, 2005

* Accepted: June 20, 2006


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