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Volume 22 |
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Number 4 |
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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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Volume 22 |
October - December 2006 |
Number 4 |
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The in vitro fertilization rate of mouse ova
in the absence or presence of recombinant
human leukemia inhibitory factorGhasem Sakiš, Zahed Safikhani2, Aligholi Sobhani3, Mojdeh Salehnia4
ABSTRACT
Objective: To examine the effect of human recombinant leukemia inhibitory factor in different doses on rate of fertilization of mouse ova.
Design: Prospective study.
Setting: Department of Anatomy, laboratory of cell culture.
Animals: Female NMRI mice 6 to 8 weeks old.
Interventions: Mice were killed at 12-14 hours after hCG or 36-38 hours after hMG injection. Mature oocytes were obtained and divided randomly into 5 groups. Oocytes in group A (n=157) were cultured as the control group in TYH medium. Oocytes in groups B, C, D, E (n=137, 154, 166 and 159, respectively) were cultured in the same medium supplemented with recombinant human leukemia inhibitory factor in four different concentrations (5, 7.5, 10, 20ng/ml, respectively) for 1 hour. After that time 100000 spermatozoa were added to every drop and after 24-26 hours two cell embryos were recorded. Fertilization was assessed by recording the number of 2-cell embryos and analysed by X2 tests.
Main outcome measurment: Two cell embryos.
Results and Conclusion: No significant difference was detected in the rate of two cell embryos in the studied experimental groups as compared with the rate of two cell embryos in control group (Group A). This study showed that, different concentrations of recombinant human of leukemia inhibitory factor in standard medium does not enhance in vitro fertilization rate of mouse oocytes.
KEY WORDS: Recombinant human leukemia inhibitory factor (rhLIF), Fertilization, Two cell embryo, NMRI mice.
Pak J Med Sci October - December 2006 Vol. 22 No. 4 438-441
1. Dr. Ghasem Saki
2. Dr. Zahed Safikhani
3. Dr. Aligholi Sobhani
4. Dr. Mojdeh Salehnia
1-2: Department of Anaomy,Faculty of Medicine,
Jondishapour University of Medical Sciences,
P.O.Box: 61335, Ahwaz, IRAN.3. Department of Anatomy, Faculty of Medicine,
Tehran University of Medical Sciences, Tehran, IRAN.4. Department of Anaomy, Faculty of Medical Sciences,
Tarbiat Modarres University,
Tehran - Iran.Correspondence:
Dr. Ghasem Saki
E-Mail: ghasemsaki@yahoo.com* Received for Publication: October 14, 2005
* Accepted: May 15, 2006
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