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Published by : PROFESSIONAL MEDICAL PUBLICATIONS |
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ISSN 1681-715X |
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ORIGINAL ARTICLE |
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Volume 25 |
October - December 2009 (Part-I) |
Number 5 |
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Vitamin-C protect ethanol induced apoptotic
neurodegeneration in postnatal rat brain
Muhammad Imran Naseer1, Najeebullah2,
Ikramullah3, Hassan Zubair4,
Mukhtiar Hassan5, Byoung Chul Yang6, Myeong Ok Kim7
ABSTRACT
Objective: To evaluate ethanol effects to induced activation of caspsae-3, and to observe the protective effects of Vitamin C (vit-C) on ethanol-induced apoptotic neurodegeneration in rat cortical area of brain.
Methodology: Administration of a single dose of ethanol in 7-d postnatal (P7) rats triggers activation of caspase-3 and widespread apoptotic neuronal death. Western blot analysis, cells counting and Nissl staining were used to elucidate possible protective effect of vit-C against ethanol-induced apoptotic neurodegeneration in brain.
Results: The results showed that ethanol significantly increased caspase-3 expression and neuronal apoptosis. Furthermore, the co-treatment of vit-C along with ethanol showed significantly decreased expression of caspase-3 as compare to control group.
Conclusion: Our findings indicate that vit-C can prevent some of the deleterious effect of ethanol on developing rat brain when given after ethanol exposure and can be used as an effective protective agent for Fetal Alcohol Syndrome (FAS).
KEY WORDS:
Ethanol, Neurodegeneration, Vitamin C, Cortex.Pak J Med Sci October - December 2009 (Part-I) Vol. 25 No. 5 718-722
How to cite this article:
Naseer MI, Najeebullah, Ikramullah, Zubair H, Hassan M, Yang BC, et al. Vitamin-C protect ethanol induced apoptotic neurodegeneration in postnatal rat brain. Pak J Med Sci 2009;25(5):718-722.
1. Muhammad Imran Naseer,
2. Najeebullah,
* M. Imran Naseer & Najeebullah contributed
equaly to this work.
3. Ikramullah,
4. Hassan Zubair,
Sheffield Hallam University,
Sheffield, S1 1WB, United Kingdom
5. Mukhtiar Hassan
Hazara University Mansehra N.W.F.P. Pakistan
6. Byoung Chul Yang,
National Institute of Animal Science,
RDA Suwan, South Korea
7. Myeong Ok Kim,
1-3,7: Division of Life Science,
College of Natural Sciences and Applied Life Science
(Brain Korea 21), Gyeongsang National University,
Gazwa 351-301, Chinju 660-701, Republic of Korea.
Correspondence:
Prof. Myeong Ok Kim, Ph.D
E-mail: mokim@gsnu.ac.kr
* Received for Publication: August 4, 2009
* Accepted: September 15, 2009
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